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Special pathology services

Pathologist analysis

TMA construction	Immunohisto- chemistry	Use of equipment	Cryostat training	Special stains	Pathologist analysis

PATHOLOGIST ANALYSIS

Pathology verification: to ensure presence of diagnostic tissue within the section, estimate semi-quantitatively amount of diagnostic material (i.e. % tumor cells), % normal adjacent tissue, % necrosis, stroma etc. This service includes generating of H&E slide if not available. For research projects that would require paraffin-embedded tissue from the surgical pathology archives, researchers who are not members of the Department of Pathology are strongly encouraged to find a collaborator among expert pathologists (Faculty). This is the best way to ensure that clinically relevant material is not exhausted and that enough tissue remains for possible future translational studies, clinical consultations and research studies.

Manual scoring: more labor-intensive review of tissues and performing scoring of morphological features, staining intensities (IHC), patterns, signal localization using either custom designed or previously tested/published scoring templates.

Automated image analysis: procedures involving slide scanning (40x, 100x, 200x), reviewing and quantitation of staining intensities, % positivity, object counting using an Automated Cellular Image System (ACIS, Clarient, USA). The slide scoring applications also include microvascular density counting, ploidy analysis, TMA scoring, integrated optical density analysis, rare cell detection and morphometric analysis based on color discrimination. For more details and feasibility studies please contact Dr. Maria Tretiakova (PIA).

Please note:

1. Any research project involving human tissues needs to have IRB approval.
2. Under no circumstances will diagnostic paraffin blocks be handed over from the surgical pathology archives to researchers outside the department of pathology. In the interests of patient care, the department has an obligation to ensure that some diagnostic tissue remains in the paraffin blocks permanently.
3. Request for TMA construction or thick slices of tissue for DNA/RNA isolation could be denied if the HTRC staff determines that this would exhaust diagnostic material for patient care purposes.