Pathology Image Analysis (PIA)

Sequence of Operation


Slide preparation, bar coding	Entry of slides data	Scanning	Review and regions selection	Automated scoring and analysis	Export of results and images

Histological slides should be clean, ideally without staining and coverslipping artifacts such as air-bubbles, folds, and background staining.
Tissue should be cut with a new blade, uneven tissue can cause focus failures
Coverslipping: If the manual coverslipping technique is used, be sure not to use excessive mounting medium
Coverslips should be cleaned with alcohol and lens cleaning paper just prior to running on the imaging system
Barcodes are provided in convenient rolls. There are 1000 duplicate barcodes per roll. Two identical labels appear adjacent to each other on the roll.
One barcode should be placed on each slide, on the right hand upper corner, with the number facing outward
The laser reads barcode lines which are horizontal, therefore pre-existing labels on the slide which are vertical will not be detected by the internal laser scanner
The additional barcode should be placed on the worksheet in the appropriate position
Positive Controls: a known positive control may be placed on the same slide as the patient/sample tissue, and/or a known positive control may be placed on a separate slide.
Negative Controls; an additional slice of tissue is cut from the patient/sample block and stained with an isotope antibody and/or a known negative tissue may be used.

The Slide Carriers

4 Slides can be placed in a slide carrier, 25 carriers can be stacked in the input hopper
Check the carriers before use to ensure they are intact, not warped or broken in any way
Slides should be placed in the carrier under the bottom tab, push down on the spring and secure firmly under the top tab
The slide carrier should be on top of the wheel in the input hopper
Do not label the slide carriers with pen, pencils or labels in the area above the slides