Welcome to the Human Tissue Resource Center. The mission of this CAP-accredited core facility is to provide investigators with a centralized infrastructure to optimize the efficiency and costs related to research involving biospecimens. The HTRC occupies 4000 square feet of remodeled space within Pathology and functions as an integrated unit. Thus, duplication of personnel, equipment, and information systems are avoided, and coordination of activities is assured.

    The HTRC now comprises four integrated facilities: Biospecimen Bank (BSB), Laser Capture Microdissection (LCM), Digital Pathology and Histology (including immunohistochemistry).

    Currently the HTRC provides services for the collection of clinically-annotated human tissues, DNA, PBMC, saliva, and other requested body fluids. In addition, the core also provides services for tissue microarray (TMA) construction as well as cryostats and digital imaging for investigators use. In this way, the HTRC provides a coordinated, centralized, and dedicated program for the procuring, processing, dispersing and assessing all types of biospecimens together with downstream histology services.


    Histology Pandemic Update

    Due to the Covid-19 Pandemic, we are now asking all users to please email us at Histology@bsd.uchicago.edu  to schedule times for dropping off and picking up projects as we are trying to limit the # of people/time spent in our lab in order to comply with current guidelines and our resumption plan.  This would also allow us to better provide our users with contactless service.  Thank you for your cooperation and understanding during this time.

    Aperio Updates

    There is a new version of ImageScope available for download. Version 12.4 contains bug fixes and general updates. Please review installation instructions and download the newest version of ImageScope.

    Aperio Quantitative Analysis Software training is now offered by Dr. Christopher Weber, Assistant Professor of Pathology. Please email Dr. Weber to schedule Aperio Analysis training.

    Turn-Around Time for Histology Services Now Shorter!

    HTRC has added additional team member which has decreased the turn-around from 3-4 weeks to 1-2 weeks on average.

    Technology Update: RNA In Situ Hybridization - 2 Additional RNAscope ISH Assays Optimized

    We have optimized two additional RNAscope ISH Assays, RNAscope 2.5 LS Red reagent and RNAscope 2.5LS Duplex Reagent, and we are ready to offer the services to our community. These two Assays can be run on Leica Bond RX automatic stainer for single or dual RNA probe detection for light microscopic observation. 10% neutral buffered formalin fixed and paraffin embedded human and mouse tissues can be used for these assays. A photo taken from a mouse kidney (FFPE) stained with RNAscope 2.5 LS Red reagent kit for a single RNA probe detection is shown below.


    Up to 4 RNA probes can be visualized using the Leica Bond RX autostainer. To learn more about using this technique, email Terri Li.

    LCM Billing Protocol Update:

    As of November 20th, the HTRC will change the method in which it bills for use of the LCM. Billing will now be based off of the time reserved via the HTRC’s scheduler. The scheduler will be compared with computer logs to ensure that the scheduler is being utilized and that all LCM use is being captured.

    If you need to cancel a reservation, we ask that you do so in advance, in order to give other users the opportunity to utilize the equipment. To cancel a reservation, please log into the scheduler prior to your reservation’s start time. Simply view your reservation and click DELETE.

    LabVantage Update:

    LabVantage has recently been upgraded to include new reporting features.

    1. Large requests will now come with a sample manifest that will list the box number and specific position for easier location of samples.
    2. It is now easier to search for paired tumor and normal samples through a new search by query option.
    3. Increased reporting is now available for participant consent data.

    Updated user manuals are available on the forms/manual page.
    Please email labvantage@bsd.uchicago.edu with any questions or comments.


    Acknowledgement of core facility contributions in publications is appreciated.

    Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.

    Sample language to acknowledge the core: We thank The University of Chicago Human Tissue Resource Center (RRID:SCR_019199), especially [staff name], for their assistance with [service name].