Welcome to the Human Tissue Resource Center. The mission of this CAP-accredited core facility is to provide investigators with a centralized infrastructure to optimize the efficiency and costs related to research involving biospecimens. The HTRC occupies 4000 square feet of remodeled space within Pathology and functions as an integrated unit. Thus, duplication of personnel, equipment, and information systems are avoided, and coordination of activities is assured.
The HTRC now comprises four integrated facilities: Biospecimen Bank (BSB), Laser Capture Microdissection (LCM), Digital Pathology and Histology (including immunohistochemistry).
Currently the HTRC provides services for the collection of clinically-annotated human tissues, DNA, PBMC, saliva, and other requested body fluids. In addition, the core also provides services for tissue microarray (TMA) construction as well as cryostats and digital imaging for investigators use. In this way, the HTRC provides a coordinated, centralized, and dedicated program for the procuring, processing, dispersing and assessing all types of biospecimens together with downstream histology services.
Turn-Around Time for Histology Services Now Shorter!
HTRC has added additional team member which has decreased the turn-around from 3-4 weeks to 1-2 weeks on average.
Technology Update: RNA In Situ Hybridization - 2 Additional RNAscope ISH Assays Optimized
We have optimized two additional RNAscope ISH Assays, RNAscope 2.5 LS Red reagent and RNAscope 2.5LS Duplex Reagent, and we are ready to offer the services to our community. These two Assays can be run on Leica Bond RX automatic stainer for single or dual RNA probe detection for light microscopic observation. 10% neutral buffered formalin fixed and paraffin embedded human and mouse tissues can be used for these assays. A photo taken from a mouse kidney (FFPE) stained with RNAscope 2.5 LS Red reagent kit for a single RNA probe detection is shown below.
Up to 4 RNA probes can be visualized using the Leica Bond RX autostainer. This image is of a formalin fixed paraffin embedded xenograft section stained with the RNAscope LS multiplex fluorescent kit from ACD with the Opal 7-color FIHC kit from PerkinElmer. Four fluorescent RNA probes plus a blue nuclear counterstain are shown. To learn more about using this technique, email Terri Li.
LCM Billing Protocol Update:
As of November 20th, the HTRC will change the method in which it bills for use of the LCM. Billing will now be based off of the time reserved via the HTRC’s scheduler. The scheduler will be compared with computer logs to ensure that the scheduler is being utilized and that all LCM use is being captured.
If you need to cancel a reservation, we ask that you do so in advance, in order to give other users the opportunity to utilize the equipment. To cancel a reservation, please log into the scheduler prior to your reservation’s start time. Simply view your reservation and click DELETE.
LabVantage has recently been upgraded to include new reporting features.
1. Large requests will now come with a sample manifest that will list the box number and specific position for easier location of samples.
2. It is now easier to search for paired tumor and normal samples through a new search by query option.
3. Increased reporting is now available for participant consent data.
Updated user manuals are available on the forms/manual page.
Please email firstname.lastname@example.org with any questions or comments.
Acknowledgement of core facility contributions in publications is appreciated.
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.